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SRX755994: Chestnut Blight QTL BAC Sequencing - Pool D
1 LS454 (454 GS FLX) run: 258,019 spots, 332.2M bases, 686.8Mb downloads

Design: Sequencing of a pool of bacterial artifical clones selected from Chinese chestnut (Castanea mollissima) BAC libraries, pool D. The BACs were picked and DNA extracted. DNA was prepared as a single end library and sequenced with ½ slide of 454 single end pyrosequencing on a GS FLX (Roche). BAC address list: CMCMBB011G13 CMCMBB021H11 CMCMBB021I10 CMCMBB024H18 CMCMBB026H16 CMCMBB027J17 CMCMBB048M12 CMCMBB056G07 CMCMBB078M24 CMCMBB080O03 CMCMBB083F01 CMCMBB085N08 CMCMBB121B23 CMCMBB153E04 CMCMBB157E04 CMCMBB178O02 CMCMBD034N07 CMCMBD059E17 CMCMBD060D21 CMCMBD060L24 CMCMBD071P02 CMCMBD077J09 CMCMBD100D22 CMCMBD102M13 CMCMBD107C15 CMCMBD112K20 CMCMBD113N12 CMCMBD120I19 CMCMBD122O16 CMCMBD127M15 CMCMBD131C20 CMCMBD133J09 CMCMBD136H04 CMCMBD139E22 CMCMBD149G01 CMCMBD150E03 CMCMBD150E17 CMCMBD154K07 CMCMBD162A15 CMCMBD170M03 CMCMBD179A08 CMCMBD185L02 CMCMBD187D05 CMCMBD193L21 CMCMBD194K24 CMCMBD195B22 CMCMBD200K22 CMCMBD206A23 CMCMBD215C04
Submitted by: University of Tennessee
Study: Castanea mollissima strain:Vanuxem Targeted Locus (Loci)
PRJNA266703 • SRP049640 • All experiments • All runs
show Abstracthide Abstract
The integrated genetic and physical map for Chinese chestnut was utilized to identify bacterial artificial clones (BACs) located in the three previously identified QTL regions conferring blight resistance. The clones were pooled and sequenced. The BAC libraries publicly available through the Clemson University Genomics Institute (www.genome.clemson.edu)
Sample: Plant sample for Castanea mollissima
SAMN02640765 • SRS590911 • All experiments • All runs
Organism: Castanea mollissima
Library:
Name: CCPoolD
Instrument: 454 GS FLX
Strategy: WGS
Source: GENOMIC
Selection: unspecified
Layout: SINGLE
Spot descriptor:
         5  forward

Runs: 1 run, 258,019 spots, 332.2M bases, 686.8Mb
Run# of Spots# of BasesSizePublished
SRR1646592258,019332.2M686.8Mb2014-11-26

ID:
1092885

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